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Caffeine in hair

Posted on October 31, 2012November 4, 2012 By Dr Tom Bassindale

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Last year I had an undergraduate student investigate the analysis of caffeine in hair samples. The aim was to determine if there was a correlation between the amount of caffeine in the hair sample and the amount of caffeine drunk by the individual.

The student produced a very good project with a fully validated method. The method analysed caffeine and three metabolites using HPLC with a UV detector. The student analysed twenty hair samples for caffeine and metabolites and plotted this versus the caffeine intake. There was a definite trend but with such a small data set it was not possible to draw further conclusions.

This year I have a student working up a method for the same topic but using an LC-MS method. Once this method is up and running we hope to analyse a much larger sample number allowing us to draw some conclusions. Watch this space for updates!

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Comments (3)

  1. Ms B says:
    November 7, 2012 at 1:23 pm

    I reckon you could make a decent cappuccino using a sample of my hair πŸ˜‰

    Reply
  2. Sue Elliott says:
    November 26, 2014 at 9:19 pm

    Could you please e mail me the amount of hair taken per analysis ?

    Reply
    1. Avatar photo Dr Tom Bassindale says:
      January 20, 2015 at 10:59 am

      Hair analyses can be conducted on anywhere from 10mg to 100mg of hair.
      Thanks
      Tom

      Reply

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